Authors: Arathi Rajan, Anuja S. Nair, Vinod Soman Pillai, Binod Kumar, Anupama R. Pai, Bimitha Benny, Mohanan Valiya Veettil

Year: 2024

Journal: Heliyon

DOI: 10.3198/heliyon.31905

Summary#

This paper synthesizes HiBiT-tagged Nipah virus-like particles for in vitro BSL-2 handling and rapid antibody neutralization studies.

Key Findings#

• The study proposes a simple approach to generate substantial amounts of HiBiT-tagged NiV-VLPs in HEK293T cells.
• These VLPs are functionally identical to the native virus and can be used for viral binding, entry, and antibody neutralization assays.
• The paper reports that the HiBiT-tag permits quick application of these particles in BSL-2 laboratories.

Methodology#

• Study Type: Experimental Study
• Sample Size: HEK293T cells

Topics#

Virology, Molecular Biology

Relevance#

The study offers a safe and effective platform for viral-based antibody neutralization assays in vitro, which can contribute to the development of targeted therapies or particle-based vaccines for Nipah virus.

Source#

[Download PDF](/pdfs/public-health-policy/main (1).pdf)