Authors: Anastasiia Kirichenko, Ekaterina Bryushkova, Vladimir Dedkov, Anna Dolgova
Year: 2023
Journal: Biosensors
DOI: 10.3390/bios13020252
Summary#
This paper describes the development of a fluorescent biosensor for detecting Nipah virus RNA using DNAzyme 10–23.
Key Findings#
- Developed a Dz_NiV biosensor based on a split catalytic core of deoxyribozyme 10–23
- Assembly of active 10–23 DNAzymes occurred only in the presence of synthetic target Nipah virus RNA
- Achieved a 10 nM limit of detection for the synthetic target RNA
Methodology#
- Study Type: Laboratory Study
Topics#
Biosensors, Virology, RNA
Relevance#
The development of this biosensor could potentially simplify and improve the detection of Nipah virus RNA, which is crucial for diagnosing and controlling outbreaks.