Authors: Sreelekshmy Mohandasa, Anita Shetea, Prasad Sarkalea, Abhinendra Kumara, Chandrasekhar Moteb, Pragya Yadava
Year: 2023
Journal: VIRULENCE
DOI: 10.1080/21505594.2023.2224642
Summary#
This paper analyzes the genomic characterization, transcriptome, and pathogenicity of a Nipah virus (Indian isolate) using Vero (ATCC® CCL−81™) and BHK−21 cells in a Syrian hamster model.
Key Findings#
- The Indian isolate shows ~4% nucleotide and amino acid difference compared to the Bangladesh strains of NiV, with most substitutions not being significant except for the phosphoprotein gene.
- Differential expression of viral genes was observed following infection in Vero (ATCC® CCL−81™) and BHK−21 cells.
- Intraperitoneal infection resulted in a dose-dependent multisystemic disease with prominent vascular lesions in lungs, brain, kidney, and extra vascular lesions in the brain and lungs.
Methodology#
- Study Type: Experimental (using cell lines and animal model)
- Geographic Focus: India
Topics#
Virology, Pathology, Epidemiology
Relevance#
The study provides insights into the genetic differences between Indian and Bangladesh Nipah virus isolates, shedding light on potential differences in pathogenicity and disease characteristics.