Nipah Open Library

Highly sensitive and quantitative HiBiT-tagged Nipah virus-like particles: A platform for rapid antibody neutralization studies

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Authors: Arathi Rajan, Anuja S. Nair, Vinod Soman Pillai, Binod Kumar, Anupama R. Pai, Bimitha Benny, Mohanan Valiya Veettil

Year: 2024

Journal: Heliyon

DOI: 10.3198/heliyon.31905

Summary

This paper synthesizes HiBiT-tagged Nipah virus-like particles for in vitro BSL-2 handling and rapid antibody neutralization studies.

Key Findings

  • The study proposes a simple approach to generate substantial amounts of HiBiT-tagged NiV-VLPs in HEK293T cells.
  • These VLPs are functionally identical to the native virus and can be used for viral binding, entry, and antibody neutralization assays.
  • The paper reports that the HiBiT-tag permits quick application of these particles in BSL-2 laboratories.

Methodology

  • Study Type: Experimental Study
  • Sample Size: HEK293T cells

Topics

Virology, Molecular Biology

Relevance

The study offers a safe and effective platform for viral-based antibody neutralization assays in vitro, which can contribute to the development of targeted therapies or particle-based vaccines for Nipah virus.

Source

View the entire paper: File:Main (1).pdf

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