Reproducible generation of Nipah virus pseudovirions with uniform incorporation of F and G surface glycoproteins for high-throughput neutralization assays
Authors: Geetu Rose Varghese, Vivek Vijay, Sreeja Sreedevi, Santhik S. Lupitha, Priya Prabhakaran, Sushama Aswathyraj, Anitha P. Moorkoth, Niyas K. Pulloor, Easwaran Sreekumar
Year: 2025
Journal: BMC Microbiology
DOI: 10.1186/s12866-025-04375-9
Summary
The paper describes the development of a system to generate Nipah virus pseudovirions with uniform incorporation of F and G surface glycoproteins for high-throughput neutralization assays.
Key Findings
- Established HEK293 pseudovirion producer cells that stably co-incorporated NiV F and G proteins
- FACS analysis of clonally selected cells for high and uniform level F and G protein co-incorporation
- Generation of high titer vesicular stomatitis virus (VSV)-based pseudoviruses from these producer cells
- Neutralization of Nipah pseudovirions by commercial anti-NiV F and G antibodies as well as convalescent serum from Nipah recovered patients
Methodology
- Study Type: Experimental
- Sample Size: Cells
- Geographic Focus: India
Topics
Virology, Molecular Biology
Relevance
The study will aid large-scale human and animal serosurveillance studies in Nipah endemic regions as well as screening of virus entry inhibitors and monoclonal antibodies.
Source
View the entire paper: File:12866 2025 Article 4375.pdf