Genomic characterization, transcriptome analysis, and pathogenicity of the Nipah virus (Indian isolate)
Authors: Sreelekshmy Mohandasa, Anita Shetea, Prasad Sarkalea, Abhinendra Kumara, Chandrasekhar Moteb, Pragya Yadava
Year: 2023
Journal: VIRULENCE
DOI: 10.1080/21505594.2023.2224642
Summary
This paper analyzes the genomic characterization, transcriptome, and pathogenicity of a Nipah virus (Indian isolate) using Vero (ATCC® CCL−81™) and BHK−21 cells in a Syrian hamster model.
Key Findings
- The Indian isolate shows ~4% nucleotide and amino acid difference compared to the Bangladesh strains of NiV, with most substitutions not being significant except for the phosphoprotein gene.
- Differential expression of viral genes was observed following infection in Vero (ATCC® CCL−81™) and BHK−21 cells.
- Intraperitoneal infection resulted in a dose-dependent multisystemic disease with prominent vascular lesions in lungs, brain, kidney, and extra vascular lesions in the brain and lungs.
Methodology
- Study Type: Experimental (using cell lines and animal model)
- Geographic Focus: India
Topics
Virology, Pathology, Epidemiology
Relevance
The study provides insights into the genetic differences between Indian and Bangladesh Nipah virus isolates, shedding light on potential differences in pathogenicity and disease characteristics.
Source
View the entire paper: File:KVIR 14 2224642.pdf