Development of a culture-independent whole-genome sequencing of Nipah virus using the MinION Oxford Nanopore platform
Authors: Md. Mahfuzur Rahman, Mojnu Miah, Mohammad Enayet Hossain, Samiur Rahim, Sharmin Sultana, Syed Moinuddin Satter, Ariful Islam, Shannon L. M. Whitmer, Jonathan H. Epstein, Christina F. Spiropoulou, John D. Klena, Tahmina Shirin, Joel M. Montgomery, Maria E. Kaczmarek, Mohammed Ziaur Rahman, Iqbal Kabir Jahid
Year: 2024
Journal: Microbial Genetics | Methods and Protocols
DOI: 10.xxxx/xxxxx or null
PMID: PMID or null
Summary
This study developed a culture-independent, high-throughput sequencing protocol for Nipah virus using the Oxford Nanopore Technology platform, suitable for resource-limited settings.
Key Findings
- Developed a WGS protocol for NiV in 24 hours
- Achieved >95% coverage of the NiV genome with an average quality score of 10.2
- Suitable for resource-limited settings, aiding in real-time monitoring, outbreak investigation, and detection of new NiV strains
Methodology
- Study Type: Development Study
- Sample Size: 12 samples (11 clinical, one environmental)
- Geographic Focus: Not specified
- Time Period: Not specified
Topics
Virology, Technology, Biosafety
Relevance
The development of this protocol is significant for NiV research as it provides a biologically safe and efficient method for whole-genome sequencing in resource-limited settings, enhancing outbreak response and surveillance.
Source
View the entire paper: File:Spectrum.02492-24.pdf