Streamlined detection of Nipah virus antibodies using a split NanoLuc biosensor

Authors: Éric Bergeron, Cheng-Feng Chiang, Michael K. Lo, Elif Karaaslan, Syed Moinuddin Satter Mohammed Ziaur Rahman, Mohammad Enayet Hossain, Wasik Rahman Aquib, Dewan Imtiaz Rahman, Subyeta Binte Sarwar, Joel M. Montgomery, John D. Klena, Christina F. Spiropoulou

Year: 2024

Journal: Emerging Microbes & Infections

DOI: 10.1080/22221751.2024.2398640

Summary

Researchers developed and validated a split NanoLuc luciferase NiV glycoprotein (G) biosensor for detecting antibodies in clinical and animal samples. The assay was tested using the WHO’s first international standard for anti-NiV antibodies and more than 700 serum samples from Bangladesh, showing sensitivity and specificity comparable to anti-NiV IgG ELISA performance.

Key Findings

The developed assay can detect antibodies in clinical and animal samples
Anti-NiV antibodies persisted for at least 8 years according to both ⍺NiV-G mix-and-read and NiV neutralization assays
The sensitivity (98.6%) and specificity (100%) of the anti-NiV-G biosensor were comparable to anti-NiV IgG ELISA performance

Methodology

Study Type: Experimental
Sample Size: More than 700 serum samples
Geographic Focus: Bangladesh

Topics

Nipah virus, serology, disease surveillance, antibodies, paramyxoviridae

Relevance

The anti-NiV-G biosensor could support the expansion of NiV surveillance and retrospective outbreak investigations.

Source

View the entire paper: File:TEMI 13 2398640.pdf