Structural and functional analysis of the Nipah virus polymerase complex

Authors: Side Hu, Heesu Kim, Pan Yang, Zishuo Yu, Barbara Ludeke, Shawna Mobilia, Junhua Pan, Margaret Stratton, Yuemin Bian, Rachel Fearns, Jonathan Abraham

Year: 2025

Journal: Cell

DOI: 10.1016/j.cell.2024.12.021

Summary

The paper determines the cryoelectron microscopy (cryo-EM) structure of the Nipah virus polymerase complex and performs structural, biophysical, and functional analyses to understand features critical for RNA replication and transcription. The findings could aid in the development of antivirals.

Key Findings

Cryo-EM structure of the NiV L-P complex determined
Docking studies with an inhibitor clarify mechanisms of intrinsic NiV L resistance
Palm insert, zinc fingers, and P4 extension are critical for NiV L activity
Intrusion loop plays an essential role in RNA replication

Methodology

Study Type: Structural analysis/Functional analysis

Topics

Virology, Pharmacology

Relevance

The paper's findings have implications for understanding Nipah virus replication and developing antivirals.

Source

View the entire paper: File:Main (2).pdf